Nitrous oxide inactivates vitamin B12 within 30 minutes and it lasts up to 3 days. Up to 3 days because the inactivation is chemically irreversible.
Everyone who is exposed to nitrous oxide on a routine basis is at risk to develop serious long-term consequences of vitamin B12 inactivation. Nerve and spinal cord damage, low blood counts. The risk of spontaneous abortion and congenital malformation is increased.
This was the reason why we designed a vitamin supplement for healthcare professionals who administer nitrous oxide on a routine basis: NitroVitamin.